产品应用

相关产品

相关产品资讯

您现在的位置:首页 > 产品应用 > 典型案例 > 典型案例

文献速递 | 作用光光质对叶绿素荧光参数ETR的影响

来源:力高泰 发布时间:2017-09-12 17:16:26 点击数: 使用提问咨询价格

LI-6800

近日,Plant and Cell Physiology期刊上发表了由John R. Evans(澳大利亚国立大学著名学者),Patrick B. Morgan(LI-COR科学家),Susanne von Caemmerer(澳大利亚国立大学著名学者)联合撰文,题为“Light Quality Affects Chloroplast Electron Transport Rates Estimated from Chl Fluorescence Measurements”的研究论文。本论文在叶绿素荧光参数准确获取方面又取得了重要进展

 

通过叶绿素荧光测量,可计算电子传递速率ETR。LI-6800便携式光合作用测量系统可同步采集气体交换和叶绿素荧光参数。研究者们通过实测叶绿素荧光参数,计算了电子传递速率ETR,并且,在抑制光呼吸的条件下测量了叶片的气体交换参数。结果发现,如果作用光是蓝光,会高估电子传递速率ETR近两倍,而选用红光则不存在这种情况。本项研究结果与“多层叶片模型”的预测结果一致。因此,在联合气体交换和荧光测量参数进行数据分析时(例如,计算叶绿体内CO2浓度),需要谨慎。

 

Light Quality Affects Chloroplast Electron Transport Rates Estimated from Chl Fluorescence Measurements

John R. Evans Patrick B. Morgan Susanne von Caemmerer

Plant and Cell Physiology, pcx103, https://doi.org/10.1093/pcp/pcx103

 

Abstract

Chl fluorescence has been used widely to calculate photosynthetic electron transport rates. Portable photosynthesis instruments allow for combined measurements of gas exchange and Chl fluorescence. We analyzed the influence of spectral quality of actinic light on Chl fluorescence and the calculated electron transport rate, and compared this with photosynthetic rates measured by gas exchange in the absence of photorespiration. In blue actinic light, the electron transport rate calculated from Chl fluorescence overestimated the true rate by nearly a factor of two, whereas there was closer agreement under red light. This was consistent with the prediction made with a multilayer leaf model using profiles of light absorption and photosynthetic capacity. Caution is needed when interpreting combined measurements of Chl fluorescence and gas exchange, such as the calculation of CO2partial pressure in leaf chloroplasts.

 


Plant and Cell Physiology期刊原文链接                                    SCI-hub.io原文链接